Functional Analysis and Classification of Phytoplankton Based on Data from an Automated Flow Cytometer.
Anthony MALKASSIAN, David NERINI, Mark A. van DIJK, Melilotus THYSSEN, Claude MANTE, and Gerald GREGORI.
Flow cytometry is a useful tool to analyse phytoplankton communities in the ocean. By measuring the light scatter, diffraction and fluorescence property on each particle, this provides a fingerprint which enables groups to be separated. A new flow cytometer called Cytosub has been developed for autonomous in situ sampling, allowing us to follow phytoplankton dynamics at short (...)

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Document from Pep Gasol
This manual contains instructions for the routine use of the FACScalibur for counting bacterial and picoalgal concentrations in natural samples. Part of the instructions (like turning on and off or cleaning the machine) will be useful to users of the machine for other applications. However, any user that intends to develop new methods or approximations or that will work with unusual samples will have to manage her/himself on his/her own. I recommend that in such a case, the cytometer manual, and a good cytometry book (like Shapiro’s “Practical Flow (...)

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Une des possibilité pour conserver un échantillons est de le fixer au PFA avant de le congeler. Cette fixation est compatible avec l’analyse ulterieure en cytométrie en flux
Preparation de 100 ml de Solution mère à 20% (200 échantillons environ)
 !!!!PRODUIT TOXIQUE – A MANIPULER SOUS HOTTE + GANTS + MASQUE
1) Dissoudre 20 g de PFA en poudre dans 100 ml d’eau bidistillée ou ultra pure
Hydratation 2 - 3 minutes
Agitation 5 minutes
2) Chauffer 30 - 60 minutes dans une étuve (80-90°C) ou 30 minutes sur une plaque chauffante (ATTENTION : éviter l’ébullition)
3) Ajouter quelques (...)

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