Auguet JC, Montanie H, Hartmann HJ, Lebaron P, Casamayor EO, Catala P, Delmas D (2009) Potential effect of freshwater virus on the structure and activity of bacterial communities in the Marennes-Oleron Bay (France). Microb Ecol 57:295-306
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Batch culture experiments using viral enrichment were conducted to test the response of a coastal bacterial community to autochthonous (i.e., co-existing) or allochthonous riverine viruses. The effects of viral infections on bacterial (...)

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Abboudi M, Surget SM, Rontani JF, Sempere R, Joux F (2008) Physiological alteration of the marine bacterium Vibrio angustum S14 exposed to simulated sunlight during growth. Curr Microbiol 57:412-417
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Growth experiments on the marine bacterium Vibrio angustum S14 were conducted under four light conditions using a solar simulator : visible light (V), V + ultraviolet A (UV-A), V + UV-A + UV-B radiation, and dark. Growth was inhibited mainly by UV-B and slightly by UV-A. UV-B (...)

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Achilles J, Stahl F, Harms H, Muller S (2007) Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression. Nat Protoc 2:2203-2211
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Characterizing and understanding the functional heterogeneity in a given population on the cellular and molecular level is a great challenge in microbiology. Each microorganism contributes differently to the overall performance of the community and responds (...)

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Achilles J, Harms H, Muller S (2006) Analysis of living S. cerevisiae cell states—a three color approach. Cytometry A 69:173-177
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BACKGROUND : Biosyntheses often fluctuate with the state of the cell in the cell cycle and on the capacity of the cell to access and metabolize a carbon source. Visualization of substrate uptake by individual cells, together with the simultaneous analysis of proliferation activity and the proportion of dead cells, facilitate reliable and (...)

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Aertsen A, Van Houdt R, Michiels CW (2005) Construction and use of an stx1 transcriptional fusion to gfp. FEMS Microbiol Lett 245:73-77
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Shiga toxins (Stxs), also termed Vero toxins, are cytotoxic ribosome inactivating proteins that are produced by a number of gastrointestinal pathogens and that contribute to the severity of the associated diseases. In this work, we constructed and validated a transcriptional fusion of the stx1AB promoter to the gfp reporter gene. The (...)

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Achilles J, Muller S, Bley T, Babel W (2004) Affinity of single S. cerevisiae cells to 2-NBDglucose under changing substrate concentrations. Cytometry A 61:88-98
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BACKGROUND : Saccharomyces cerevisiae is a widely employed microorganism in biotechnological processes. Since proliferation and product formation depend on the capacity of the cell to access and metabolize a carbon source, a technique was developed to enable for analyzing the S. cerevisiae H155 cells’ affinity (...)

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Abd H, Johansson T, Golovliov I, Sandstrom G, Forsman M (2003) Survival and growth of Francisella tularensis in Acanthamoeba castellanii. Appl Environ Microbiol 69:600-606
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Francisella tularensis is a highly infectious, facultative intracellular bacterium which causes epidemics of tularemia in both humans and mammals at regular intervals. The natural reservoir of the bacterium is largely unknown, although it has been speculated that protozoa may harbor it. To test this (...)

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Amadori M, Tagliabue S, Lauzi S, Finazzi G, Lombardi G, Telo P, Pacciarini L, Bonizzi L (2002) Diagnosis of Mycobacterium bovis infection in calves sensitized by mycobacteria of the avium/intracellulare group. J Vet Med B Infect Dis Vet Public Health 49:89-96
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Because of the frequent exposure of cattle to mycobacteria of the avium/intracellulare group, an investigation was carried out into the possible repercussions thereof on the diagnosis of bovine tuberculosis. Three (...)

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(2001) Flow cytometry in the marine environment. Proceedings from the 20th ISAC Congress. Montpellier, France, 20-25 May 2000. Cytometry 44:163-271
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Adachi H, Saito I, Horiuchi M, Ishii J, Nagata Y, Mizuno F, Nakamura H, Yagyu H, Takahashi K, Matsuoka T (2001) Infection of human lung fibroblasts with Epstein-Barr virus causes increased IL-1beta and bFGF production. Exp Lung Res 27:157-171 (...)

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Albanyan EA, Vallejo JG, Smith CW, Edwards MS (2000) Nonopsonic binding of type III Group B Streptococci to human neutrophils induces interleukin-8 release mediated by the p38 mitogen-activated protein kinase pathway. Infect Immun 68:2053-2060
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Nonopsonic interaction of host immune cells with pathogens is an important first line of defense. We hypothesized that nonopsonic recognition between type III group B streptococcus and human neutrophils would occur and that the (...)

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al-Majali AM, Asem EK, Lamar C, Robinson JP, Freeman J, Saeed AM (1999) Insulin modulates intestinal response of suckling mice to the Escherichia coli heat-stable enterotoxin. Adv Exp Med Biol 473:113-123
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Effect of insulin on the response of suckling mice to the enterotoxigenic Escherichia coli heat-stable enterotoxin (STa) was studied. Four groups (8-10 in each group) of two day old Swiss Webster suckling mice were used. Five, 10, 25, and 50 micrograms of insulin were (...)

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Abad FX, Pinto RM, Bosch A (1998) Flow cytometry detection of infectious rotaviruses in environmental and clinical samples. Appl Environ Microbiol 64:2392-2396
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A method for the detection of infectious human rotaviruses based on infection of CaCo-2 cells and detection of infected cells by indirect immunofluorescence and flow cytometry (IIF-FC) has been developed. The technique was validated by performing a seminested reverse transcription-PCR assay with sorted cell (...)

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Alfa MJ, DeGagne P (1997) Attachment of Haemophilus ducreyi to human foreskin fibroblasts involves LOS and fibronectin. Microb Pathog 22:39-46
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Haemophilus ducreyi is the causative agent of the genital ulcer disease Chancroid. Chancroid has been shown to increase the risk of heterosexual transmission of HIV. Little is known regarding the attachment or localization of this organism to human cells in either the dermal or epidermal layer. In this study the attachment of H. (...)

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Akerlund K, Bjork L, Fehniger T, Pohl G, Andersson J, Andersson U (1996) Sendai virus-induced IFN-alpha production analysed by immunocytochemistry and computerized image analysis. Scand J Immunol 44:345-353
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IFN-alpha production in Sendai virus-stimulated human buffy coat cultures could readily be demonstrated in individual cells at a protein level by the use of a novel immuno-enzymatic staining procedure. A distinctive rounded, juxtanuclear staining pattern was generated (...)

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Akerlund T, Nordstrom K, Bernander R (1995) Analysis of cell size and DNA content in exponentially growing and stationary-phase batch cultures of Escherichia coli. J Bacteriol 177:6791-6797
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Escherichia coli strains were grown in batch cultures in different media, and cell size and DNA content were analyzed by flow cytometry. Steady-state growth required large dilutions and incubation for many generations at low cell concentrations. In rich media, both cell size and DNA (...)

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Alvarado-Aleman FJ, Gonzalez-Bonilla C, Wong-Arambula C, Gutierrez-Cogco L, Sepulveda-Amor J, Kumate-Rodriguez J (1994) [Identification of Vibrio cholerae O1 by flow cytometry]. Rev Latinoam Microbiol 36:283-293
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A total of 72 peptonated water samples suspected of carrying Vibrio cholerae were assessed by laser flow cytometry (LFC) and compared with positive culture. We used a direct fluorescence technique using polyclonal (PolAb) and monoclonal antibodies (MoAb) (...)

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Accotto GP, Mullineaux PM, Brown SC, Marie D (1993) Digitaria streak geminivirus replicative forms are abundant in S-phase nuclei of infected cells. Virology 195:257-259
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We have analyzed the replication of Digitaria streak geminivirus (DSV) using flow cytometry. Nuclei from infected plants were sorted on the basis of their DNA content in the different phases of the cell cycle and viral DNA forms in them were analyzed. DSV replicative forms were much more abundant (up to (...)

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Aran JM, Plagemann PG (1992) Nucleoside transport-deficient mutants of PK-15 pig kidney cell line. Biochim Biophys Acta 1110:51-58
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Previous studies indicated that PK-15 pig kidney cells express solely a nitrobenzylthioinosine-sensitive, equilibrative nucleoside transporter. In the present study, PK-15 cells were mutagenized by treatment with ICR-170 and nucleoside transport-deficient mutants selected in a single step in growth medium containing tubercidin and cytosine (...)

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An GH, Bielich J, Auerbach R, Johnson EA (1991) Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting. Biotechnology (N Y) 9:70-73
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The carotenoid pigment astaxanthin (3,3’-dihydroxy-beta,beta-carotene-4,4’-dione) is an important component in feeds of aquacultural animals. It is produced as a secondary metabolite by the yeast Phaffia rhodozyma, and the isolation of rare mutants that produce increased quantities is (...)

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Bernander R, Nordstrom K (1990) Chromosome replication does not trigger cell division in E. coli. Cell 60:365-374
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An essential part of the chromosome replication origin of E. coli K-12 and B/r was replaced by the plasmid pOU71. The average initiation mass of replication for pOU71 decreases with increasing temperature. The constructed strains were grown exponentially at different temperatures, and cell sizes and DNA content were measured by flow cytometry. The average DNA (...)

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Bernander R, Merryweather A, Nordstrom K (1989) Overinitiation of replication of the Escherichia coli chromosome from an integrated runaway-replication derivative of plasmid R1. J Bacteriol 171:674-683
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A 16-base-pair fragment, deletion of which completely inactivated oriC, was replaced by a temperature-dependent runaway-replication derivative (the copy number of which increases with temperature) of the IncFII plasmid R1. The constructed strains were temperature (...)

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Alderete JF (1988) Alternating phenotypic expression of two classes of Trichomonas vaginalis surface markers. Rev Infect Dis 10 Suppl 2:S408-412
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The antigenic heterogeneity of Trichomonas vaginalis is due in part to the membrane disposition of immunogens (repertoire A) among some but not all isolates and among subpopulations of trichomonads of certain isolates. Heterogeneous T. vaginalis isolates undergo phenotypic variation for the A repertoire of immunogens. The (...)

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Christersson CE, Fornalik MS, Baier RE, Glantz PO (1987) In vitro attachment of oral microorganisms to solid surfaces : evaluation of a controlled flow method. Scand J Dent Res 95:151-158
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A flow cell method was modified to provide shear-controlled experimental conditions for monitoring initial attachment and detachment of oral microorganisms to solid surfaces. Whole unstimulated human saliva was collected and circulated at a flow rate of 1 ml/min, through a cell composed (...)

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Donnelly CW, Baigent GJ (1986) Method for flow cytometric detection of Listeria monocytogenes in milk. Appl Environ Microbiol 52:689-695
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This report describes a method for the detection of Listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. The use of immunofluorescence in combination with measures of DNA content by propidium iodide labeling and size by light scattering enabled specific identification of L. (...)

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Mansour JD, Robson JA, Arndt CW, Schulte TH (1985) Detection of Escherichia coli in blood using flow cytometry. Cytometry 6:186-190
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A rapid method for the detection of Escherichia coli in blood has been developed. The method employs blood cell lysis, staining of bacteria with ethidium bromide, and detection of stained bacteria using flow cytometry. The detection protocol requires less than 2 h sample handling time and is not dependent on bacterial growth. This method has (...)

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Barnett JM, Cuchens MA, Buchanan W (1984) Automated immunofluorescent speciation of oral bacteria using flow cytometry. J Dent Res 63:1040-1042
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Mixtures containing two bacterial species were analyzed using flow cytometric techniques. Light scattering characteristics of Streptococcus mutans and Actinomyces viscosus show unique profiles for pure cultures. However, the light scatter analysis of a mixture containing these two species demonstrates overlapping near the origin. (...)

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Boye E, Steen HB, Skarstad K (1983) Flow cytometry of bacteria : a promising tool in experimental and clinical microbiology. J Gen Microbiol 129:973-980
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The DNA and protein content of individual Escherichia coli cells were measured at a rate of 10(4) cells per second with a sensitive microscope-based flow cytometer. DNA and protein were quantified by measuring the fluorescence from cells stained with a combination of the DNA-binding drugs Mithramycin and ethidium bromide (...)

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Bezrukova AG, Vladimirskaia IK (1982) Information value of light scattering parameters in cell study (a review of the literature). Tsitologiia 24:507-521
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Different methods of light scattering measurements for cell suspension and cells in flow are reviewed. The intensity of light scattered at different angles suggests information about the cell size, the nucleus to cell diameter ratio, the thickness of surface membrane, the changes in the internal cell structure state, (...)

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Fulwyler MJ (1980) Flow cytometry and cell sorting. Blood Cells 6:173-184
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Flow cytometry has become an important research tool in cytology, genetics, immunology, and microbiology. The information gained from cytometric instruments is quantitative and of high statistical precision, enabling resolution of cell subpopulations. Although increasing, application to cytology is hindered by inadequate appreciation of the nature of flow cytometry and the information obtained. (...)

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