PRECYM : Plate-forme Régionale de Cytométrie pour la Microbiologie
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Atelier INSERM - Advances in cytometry : new applications and insights in basic and clinical research
mardi 12 mars

1-3 octobre 2013 ◘ 1 st -3 rd October 2013 Bordeaux, France Aims :

Recently, great technological improvements have been performed in multiparameter flow cytometry (MFC) to study complex biological systems. However, the difficulty of implementing MFC is a real barrier limiting its broad use. Similarly, knowledge is often lacking, in advanced analytical tools and methods needed to handle the mass of information generated by MFC. An emerging innovating technology, the multiparameter mass cytometry (MMC) allows the simultaneous analysis of more than 40 parameters. This new technology (also referred to as CyTOF) faces similar challenges in analysis and data visualization as MFC. Therefore, the formation into the laboratories is crucial to control all aspects of multiparametric approaches that will be addressed during this workshop. To fulfill these goals, MFC and MMC technologies will be introduced by experts of each experimental field and illustrated using concrete examples of applications in clinical research. These presentations will be completed by an introduction to current software solutions in order to analyze huge datasets generated by these two approaches. Finally, emerging technologies will be presented, mainly in the field of image cytometry. Audience All biological scientists, engineers, medical doctors, post-doctoral fellows and students who are interested in the application of advanced cytometry techniques in their respective experimental fields and are eager to increase the number of parameters studied simultaneously. A basic knowledge in conventional flow cytometry (4-6 parameters) is required. Lectures will be given in English.

Avec la participation de / with the participation of :
- Mario ROEDERER (Bethesda, USA),
- Francis LACOMBE (Bordeaux, France),
- J. Paul ROBINSON (Indiana, USA),
- Juan FLORES-MONTERO (Salamanca, Spain),
- Ryan R. BRINKMAN (British Columbia, Canada),
- Rodolphe THIEBAUT (Bordeaux, France),
- Andrea COSSARIZZA (Modena, Italy),
- Scott TANNER (Toronto, Canada),
- Jonathan IRISH (Nashville, USA),
- Samuel GRANJEAUD (Marseille, France),
- Garry NOLAN (Stanford, USA),
- Attila TARNOK (Leipzig, Germany),
- Philippe BOUSSO (Paris, France),
- Michael GERNER (Bethesda, USA),
- Marie FOLLO (Freiburg, Germany),
- William TELFORD (Bethesda, USA).



— Date limite d’inscription : 21 juin 2013  Registration deadline : June 21 st 2013
— Renseignements et inscriptions / Information and registration
— Inserm / Ateliers de formation / 101 rue de Tolbiac / 75654 Paris Cedex 13
— Tel. +33 (0) 1 44 23 62 04 or 62 03 / Fax +33 (0) 1 44 23 62 93 / ateliers@inserm.fr

 
Sur le Web : Atelier INSERM
Première place accordée au CNRS par le Scimago Institutions Ranking (SIR) - CNRS awarded first place at the SCImago Institutions Ranking by (SIR)
jeudi 3 janvier

Première place accordée au CNRS par le Scimago Institutions Ranking (SIR) qui produit le classement le plus complet des meilleurs institutions scientifiques mondiales.

Le CNRS figure également en 2012 parmi les 100 institutions mondiales, publiques ou privées, leaders dans le domaine de l’innovation (« Top 100 global innovators », Thomson-Reuters).

CNRS awarded first place at the SCImago Institutions Ranking by (SIR), which produces the most comprehensive ranking of the best scientific institutions worldwide.

CNRS is also in 2012 among the 100 global institutions, public and private leaders in the field of innovation ("Top 100 global innovators," Thomson-Reuters).

 
An excellent tutorial in flow cytometry : with many animations
lundi 29 octobre

This a very nice tutorial describing the verious aspects of a flow cytometer thanks to sseveral animations.

Click here

 
L’observation in situ à haute fréquence du phytoplancton à l’honneur dans le journal Le Monde
samedi 1er septembre

Un article paru dans le journal Le Monde du 25 août 2012 et intitulé : Des microalgues sous haute surveillance met à l’honneur l’’observation in situ à haute fréquence du phytoplancton.

Lien vers l’article

 
A new movie about the phytoplankton monitoring in the Bay of Villefranche (NW Mediterranean Sea)
jeudi 31 mai

This movie aims to describe the instruments installed on the buoy for this preliminary project. Some data are also presented. To watch the movie, click here This project is a collaborative project between MIO (Marseille), LOV and OOV (Villefranche-sur-mer), and LOG (Wimereux)

BoueeEOL

People involved :
- Gérald Grégori and Michel Denis (MIO)
- Melilotus Thyssen, Felipe Artigas (LOG),
- Jean-Michel Grisoni (OOV)
- Maria-Luiza Pedroti and Laure Mousseau (LOV)

Support of :
- the crew of the La Velelle (ship of the OOV)
- the support of the Service d’Observation (MIO)
- the Cytobuoy b.v. compagny (The Netherlands)
- Program EC2CO PNEC (MISE ; PI : G. Grégori)

 
Sur le Web
Plateforme Régionale de Cytométrie pour la Microbiologie PRECYM
Site de la Plateforme Régionale de Cytométrie pour la Microbiologie PRECYM
Hyperspectral Cytometry at the Single-Cell Level Using a 32-Channel Photodetector
lundi 29 août

Zoom sur un Article :

Gregori, G., Patsekina, V., Rajwa, B., Jones, J., Ragheb, K., Holdman, C., Robinson, J.P., (2011) Hyperspectral cytometry at the single-cell level using a 32-channel photodetector. Cytometry (DOI : 10.1002/cyto.a.21120)

Despite recent progress in cell-analysis technology, rapid classification of cells remains a very difficult task. Among the techniques available, flow cytometry (FCM) is considered especially powerful, because it is able to perform multiparametric analyses of single bio- logical particles at a high flow rate–up to several thousand particles per second. More- over, FCM is nondestructive, and flow cytometric analysis can be performed on live cells. The current limit for simultaneously detectable fluorescence signals in FCM is around 8–15 depending upon the instrument. Obtaining multiparametric measure- ments is a very complex task, and the necessity for fluorescence spectral overlap com- pensation creates a number of additional difficulties to solve. Further, to obtain well- separated single spectral bands a very complex set of optical filters is required. This study describes the key components and principles involved in building a next-genera- tion flow cytometer based on a 32-channel PMT array detector, a phase-volume holo- graphic grating, and a fast electronic board. The system is capable of full-spectral data collection and spectral analysis at the single-cell level.

 

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