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TEAM 1 ANALYTICAL TOOLS |
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| Sediment reworking quantification/ 1D
method |
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2D
method The non-destructive 2D approach to quantify particle reworking is a powerful and complementary tool to understand particle transport by the benthic fauna in surface sediments. The optical technique for 2-D detection of luminophores is relatively fast and easy to perform, with the ability to detect small scale (<mm) particle movements on a time resolution of minutes or less. The use of a specific numerical model allows to quantify (optical reworking coefficient: ORC) the particle movement. |
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Analyses
of Total organic carbon/ High Temperature Combustion Oxidation (HTCO). Shimadzu 5000. Have a look on DOC website: http://www.imcs.rutgers.edu/~spyres/HTCO.htm |
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Analyses
of Amino-acids/ Amino acids are derivatized (with OPA) (Lindroth and Mopper, 1979) and separated on Symmetry Waters C18, 25 cm column by using a Waters 1525 Binary HPLC pump and detected with a Fluoresence 474 Waters detector. |
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Analyses
of OH. radicals/ Benzoic acid reacts with OH. radicals to form o-hydroxybenzoic acid (OHBA) (Zhou and Mopper, 1990) which is detected by using Fluorescence 474 Waters detector after chromatographic separation by HPLC Breeze Waters 1525 binary HPLC pump and a Symmetry Waters C18, 15 cm column. |
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Analyses
of sugars/ Monosaccharides are separated on an anion exchange column (Carbopac PA-1, Dionex) by using Waters 626 quaternary pump and Waters 600 S controller and an isocratic elution with 19 mM NaOH at 17 °C (column temperature) and detected by an electrochemical detector (Decade, Antec). |
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Analyses of lipid molecules/ Analyses
of dicarboxylic acids/ Analyses of long chain fatty acids FAME analysis is performed on a Perkin Elmer Autosystem XL equipped with a split/splitless injector and with a polar BPX70 SGE column. Analytical conditions are 30 m column length, injection temperature 240°C, detection temperature 280°C, oven temperature 50-260°C. |
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Identification
of individual biomarker components/
Individual lipid biomarkers are identified with a HP 5890 series II plus gas chromatograph coupled to a HP 5972 mass spectrometer. Compounds are separated using classical capillary columns like (HP1-MS, HP5-MS, Solgel-1, BPX-50 etc.). Selective
detection of elements/ |
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Analyses
of lipid classes/ Lipid extracts are separated on chromarods and quantified on a thin layer chromatography/flame ionisation detection (TLC/FID) Iatroscan TH10 apparatus model MK-VIs (hydrogen flow, 160ml/min; air flow, 2000 ml/min) coupled to PC equipped with a Chromstar integration system (Bionis, 2001). The separation scheme involved five elution steps in solvent systems of increasing polarity: hexane + diethylether + formic acid, acetone, chloroform + methanol and chloroform + methanol + ammonium hydroxide according to a modified procedure of Goutx et al. (1990). In such conditions, 14 compounds are identified and quantified; the relative standard deviation of replicate samples (n=3) for Iatroscan TLC-FID analysis was 3 to 11%. |
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Measurements of Ultra-Violet (UV) and Photostynthetic active radiations (PAR) in lower troposphere and in seawater/
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OCR-504 I sensors/ Downwelling irradiance (in W m-2).Dose (in kJ m-2). (Photo 7 OCR 504/I). Underwater measurements : UV: 305, 325, 340, 380 nm (bandwidth: 2 and 10 nm). PAR: 412, 443, 490, 565 nm (bandwidth: 20 nm). | |||||||||||||||||
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