ENDNEL2s&9ÿÿÿÿa,TI-AU-SO-, , v. , iss. , pp. SO-' (), , , v. , iss. , pp. VO-IP-YR-DT-PG-SP-PD-LA-AB-AA-SU-MD-MC-IS-PT-AN-UC-AF-'øDD[ ] - . H / [ ] D edited by by H H: , USA [ ] , U.S.A. D[ ] , D DDD p. ; : p Dby HD*ed. version. version [ ] edition. edition HS: . [ ] / , ( ) DHD: DDDScale DDDSLSDL L!L"L#L$L%L&L'Dj ‚‚‚ ‚ÿ ‚ ‚‚‚‚‚‚‚‚‚‚‚‚‚‚ SO-jEBSCO;NO- 1 TI- Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR green I. AU- Marie, Dominique AU- Partensky, Frederic AU- Jacquet, Stephan AU- Vaulot, Daniel AF- Station Biologique, B 74, F-29682 Roscoff Cedex, France SO- Applied and Environmental Microbiology, 1997, v. 63, iss. 1, pp. 186-193. PY- 1997 AB- The novel dye SYBR Green I binds specifically to nucleic acids and can be excited by blue light (488-nm wavelength). Cell concentrations of prokaryotes measured in marine samples with this dye on a low-cost compact flow cytometer are comparable to those obtained with the UV-excited stain Hoechst 33342 (bis-benzimide) on an expensive flow cytometer with a water-cooled laser. In contrast to TOTO-1 and TO-PRO-1, SYBR Green I has the advantage of clearly discriminating both heterotrophic bacteria and autotrophic Prochlorococcus cells, even in oligotrophic waters. As with TOTO-1 and TO-PRO-1, two groups of heterotrophic bacteria (B-I and B-II-like types) can be distinguished. Moreover, the resolution of DNA distribution obtained with SYBR Green I is similar to that obtained with Hoechst 33342 and permits the analysis of the cell cycle of photosynthetic prokaryotes over the whole water column. MD- ANALYTICAL METHOD MD- CELL CYCLE MD- CELL CYCLE ANALYSIS MD- ENUMERATION METHOD MD- FLOW CYTOMETRY MD- MARINE MD- MARINE ECOLOGY MD- METHODOLOGY MD- NUCLEIC ACID STAIN MD- SYBR GREEN I MC- Biochemistry and Molecular Biophysics MC- Cell Biology MC- Marine Ecology (Ecology, Environmental Sciences) MC- Methods and Techniques MC- Microbiology OG- microplankton (Organisms - Unspecified) OG- organisms (Organisms - Unspecified) CT- 01054-02502-07512*10060-10062-29500- BC- 00500 LA- English IS- 00992240 PT- Research Article RT- Abstract AN- BIOA199799362530 arch Article RT- Abstract AN- BIOA199799362530