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Plants
are said to be autotrophic:
their organic matter is synthesized from the substances (water
and mineral salts) they extract from the soil or from the
aquatic media in which they live.
The energy required to perform this process of synthesis is
provided by the sun. This energy is captured by assimilating
pigments (chlorophylls) present in the chloroplast of plant
cells or in specialised regions of the cell membranes of
procaryotic cells (cells with no nucleus).
Photosynthesis
can be described in terms of the following general formula:
n
(CO2+H2O) + hv (Light energy)
-------->
(CH2O)n + nO2
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1)
The principle
The
structures responsible for photosynthesis form the photosystem:
this system consists of groups of several hundreds of
chlorophyll molecules surrounded by the thylakoid (a
structural unit composed of sacs and
vesicles), where the photosynthesis takes place.
In eucaryotes (organisms composed of cells with individual
nuclei), there are two kinds of photosystems: I and II (or P700
and P680, respectively). The accessory pigments absorb the light
and transport the energy from one molecule to another from the
periphery of the system to the
reaction centre,
consisting of a specialised pair of chlorophyll
a molecules.
When excited by photons, these molecules are able to produce
electron acceptor electrons.
Diagram
of a photosystem

The
electrons excited by light are then accepted by molecules
forming an electron transport chain. These reactions occurring
within the thylakoid membranes are known as “
photochemical reactions".
2)
the
light phase in the process of photosynthesis: the two types of
photochemical reactions
Cyclic
and acyclic
photophosphorylation
are both photodependent reactions.
This
is the simplest pathway taken by excited electrons.
-
ATP (the high-energy molecule Adenosine Triphosphate) is
produced, but no O2 or NADPH (the redox potential
molecule Nicotinamide adenosine diphosphate).
-
The
excited electrons leave the chlorophyll in the reaction centre,
travel along a short electron transport chain and return to the
reaction centre.
-
During a series of oxydoreduction (redox) steps,
the electron is transported from one protein to another.
-
All these processes occur within the inner thylakoid membrane.

ATP
is produced indirectly by the proton motor force (via an
electrochemical gradient) due to the transfer of the protons
from the outside to the inside of the thylakoid membrane.
This
reaction involves the two photosystems (I et II) and the reaction
centres (P700 et P680).
Upon
being excited by light energy, an electron leaves the
chlorophyll molecule in photosystem II. To compensate for this
loss, the molecule in question recovers an electron via the
photolysis of the water molecule :
H2O
---> 2 H+ + 1/2 O2 + 2e- (Photolysis
of water)
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This
results in the production of O2 and ATP (indirectly
via the proton motor force) and NADP+ is reduced to NADPH and
H+.
The water is therefore the electron donor and NADP+ is the final
acceptor;
the O2, released into the atmosphere is used for cell
respiration purposes.

The
light phases therefore convert the solar energy captured by the
pigments into chemical energy, which is stored in the
high-energy ATP molecules and the NADPH (redox potential)
molecules. ATP is therefore synthesized as the result of
the proton motor force and ATP synthetase, which triggers the
reaction ADP + Pi ---> ATP.
The formation of these two molecules favours the binding of CO2:
this is known as
the
Calvin cycle.
3)
the dark phase in the process of photosynthesis: the Calvin
cycle
The
Calvin cycle takes place in the stroma of eucaryotic
chloroplasts.
This is the final stage in the process of
photosynthesis in which the ATP and the NADPH produced
during the previous photochemical reactions are used.
This cycle consists of a series of biochemical reactions
controlled by various enzymes, which result in the reduction and
incorporation of atmospheric CO2 into the organic
molecules.
The
key enzyme in this cycle is Rubisco, which enables CO2
to bind to RuBP: Rubisco,
or ribulose-1-5-biphosphate carboxylase, accounts for
up to 16 % of all the protein present in the chloroplast; it is
one of the most indispensable and abundant proteins on the earth.
This
cycle is repeated 6 times (i.e., CO2 is incorporated
6 times), yielding one glucose molecule, for example. This
glucose will subsequently be used to synthesize polysaccharides,
fatty acids, amino acids, nucleotides and all the other
molecules on which the life of the plant depends.

Other sites:
http://gened.emc.maricopa.edu/bio/bio181/BIOBK/BioBookPS.html
(nombreux schémas explicatifs)
http://www.lbte.univ-mrs.fr/photweb.html
http://www.brunette.brucity.be/lgmlej/04SetT/04001CoupdSol/13-photosynth.htm
http://mars.rever.fr/Articles/AlguesSymbiotiques.html
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